HRP-conjugated beta Actin Antibody

Product Details


Product Information
Source Mouse Purification method Caprylic acid/ammonium sulfate precipitation
Isotype IgM Storage PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3. Store at -20oC.
Immunogen Information
Immunogen Recombinant Protein Full name actin, beta
Calculated molecular
weight
375aa,42 kDa Observed molecular
weight
42 kDa
GenBank accession
number
BC002409 Gene ID (NCBI) 60
Gene symbol ACTB Synonyms ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, HRP β actin, HRP-ACTB, HRP-Beta actin, HRP-F actin, PS1TP5BP1, β actin
Background

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

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