PKM1-specific Antibody
Product Details
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | Peptide | Full name | PKM2 pyruvate kinase, muscle |
|
Calculated molecular weight |
58 kDa |
Observed molecular
weight |
58 kDa |
|
GenBank accession number |
NM_182470 | Gene ID (NCBI) | 5315 |
| Gene symbol | PKM2 | Synonyms | CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK |
PKM2, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM2 plays a general role in caspase independent cell death of tumor cells. PKM2, Pyruvate kinase isozymes M1/M2, has 2 isoforms .The immunogen of this antibody is M1 isoform, also called PKM1. By the upstream metabolite fructose-1,6-bisphosphate, PKM1 is a constitutively active enzyme.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823) This antibody is specific to PKM1 isoform. |